IRTA-Cal
Institute for Food and Agricultural Research and Technology,
Generalitat of Catalonia
www.gencat.cat
www.irta.cat
George Bruening Laboratory
Plant Pathology Department
Hutchison Hall
University of California
Davis, CA 95616
phone (530) 752-3474
lperezgrau@ucdavis.edu
IRTA@ucdavis.edu

EDUCATION

Ph.D. (Biochemistry) 1982
University of Barcelona (Spain)

HONORS

1986-1988 Fulbright Research Scholarship, University of California, Los Angeles. CA.
1983 Woods Hole Marine Biology Fellowship to attend the Embryology Summer Course.
1982-84 European Molecular Biology Laboratory (EMBO) Postdoctoral Fellow, Hamburg, Germany.

PROFESSIONAL EXPERIENCE

2002 AgResearch and Biotech Liaison IRTA (Institut de Recerca I Tecnologia Agroalimentaries)- UC (University of California).
2002 Senior Scientist. Plant Sciences Department. J.R. Simplot Co. Boise, ID.
2000-2002 Senior Research Scientist coordinator of Genomic Research CERES-Seed Institute. Division of Plant Molecular Biology. University of California, Davis, CA.
1998-2000 Senior Scientist, Monsanto Calgene Campus, Davis, CA.
1993-1997 Principal Investigator, Calgene Inc, Davis, CA.
1997-1999 Co-Principal Investigator. CEPRAP Center for Engineering Plants for Resistance Against Pathogens. University of California. Davis, CA.
1991-1993 Research Scientist, Biotechnology, DuPont Agricultural Products, Experimental Station, Wilmington, DE.
1986-1988 Senior Research Scientist, Department of Biology, University of California, Los Angeles, CA.
1986-1988 Fulbright Research Scholar at Prof. R. B. Goldberg laboratory, Department of Biology, University of California, Los Angeles, CA.
1984-1986 Researcher, Laboratory of Molecular Genetics, Centro Investigación y Desarrollo, CSIC, Barcelona, Spain.
1982-1984 Postdoctoral Fellow European Molecular Biology Laboratory, Hamburg, West Germany.

RESEARCH PROJECTS & PUBLICATIONS

Olive trees and Verticillium

In collaboration with Prof. Tom Gordon Laboratory, Plant Pathology UC Davis, collecting isolates of Verticillium pathogens from diseased olive trees. Assesment of High Density and Super High Density olive materials for their response to California’s olive tree Verticillium pathogens.

Pepper Transformation Protocol

In collaboration with the Tissue Culture and Plant Transformation Facility at UCD we developed an efficient protocol for transforming pepper with Agrobacterium.

Pepper Fruit Improvement
Using mutagenesis and targeted selection in collaboration with Semillas Fito, IRTA and Arcadia Biosiences Inc. we produced a collection of mutant lines suitable to fingerprint by TILLING to identify pepper fruits with desirable properties in shelf life, vitamins and micronutrient content.

Cucumber Vein Yellowing Virus Elisa Test
The components for an ELISA test for the CVYV virus were developed to facilitate the detection of the CVYV virus that all but decimated the production of cucumbers in South Eastern Spain in the 2001-2002 seasons.

Genetic and Genomic Approaches to Identify Programs of Gene Expression Activated Early After Fertilization. Regulatory Genes of Embryo and Seed Development.

L. Pérez-Grau. (2001) Plant Embryogenesis. The Cellular Design of a Plant. in Plant Reproduction. S.O’ Neill and J.A. Roberts eds. Sheffield Academic Press.
J. Okamuro, D. Jofuku, R. Penell, Z. Chen, V.D. Dang, J.Donson, Y. Fang, W. Volkmuth, R.B. Flavell, J. Harada, G. Drews, A. Bui, l. Perez-Grau, R. Fisher and R.B. Goldberg (2001)
A comprehensive Analysis of Gene Expression from Ovule to Seed and from Flower to Fruit.
International Plant and Animal Genome IX Conference

Metabolic Engineering of the Carotenoid Biosynthesis in Plant Seeds. Production of Antioxidants and Natural Pigments for Human and Animal Nutrition.

L. Pérez-Grau, C. Huang, D. Ke and C. Shewmaker Metabolic Engineering of Ketocarotenoid Food Pigments Production in Plant Seeds. (In Preparation)
L. Pérez-Grau and C. Shewmaker. (1999) Carotenoids Biosynthesis Pathway. Calgene Patent Submission.
L. Pérez-Grau and C. Shewmaker. (1998) Production of Carotenoids in Plant Seeds. Calgene Patent Submission.

Metabolic Engineering of Natural Pigment Biosynthesis in Cotton for Color Fiber. Production. Construction of Fiber Cell Specific Gene Expression Cassettes.

Y. Ruan, P. Chourey, D. Delmer, and L. Pérez-Grau. (1997) Differential Expression of Sucrose Synthase in Relation to Diverse Patterns of Carbon Partitioning in Developing Cotton Seed. Plant Physiol. 115: 375-385.
J. Pear, L. Pérez-Grau and D. Stalker. (1995) Cotton Fiber Transcription Factors. U.S. Calgene Patent number 115.

Genetic Engineering of Plant Embryo Storage Products. Isolation of Genes encoding ?-Fatty Acid Desaturases from Arabidopsis, Corn, Rapeseed and Soybean to Enhance Seed Oil Quality.

W. Hitz, A. Kinney, J. Lightner, Okuley J., Pérez-Grau L., and N. Yadav. (1998) Genes for microsomal Delta-12 Fatty Acid Desaturases and Related Enzymes from Plants. US DuPont Patent.
W. Hitz, N. Yadav, and L. Pérez-Grau. (1995). Nucleotide sequence of soybean stearoyl-ACP desaturase gene. United States. Patent number 5,443.974.
N. Yadav, A. Wierzbicki, M. Aegerter, C. Caster, L. Pérez-Grau, A. Kinney, W. Hitz, J. Booth, B. Schweiger, K. Stecca, S. Allen, M. Blackwell, R. Reiter, T. Carlson, S. Russell, K. Feldman, J. Pierce, and J. Browse. (1993). Cloning of higher plants ?-3 fatty acid desaturases. Plant Physiol. 103, 467-476.
J.Browse, L. Pérez-Grau, A. Kinney, J. Pierce, A. Wierzbicki and N. Yadav. (1992). Fatty acid desaturase genes from plants. U.S. DuPont Patent BB-1036-A.

Spatial Patterns of Gene Expression and Molecular Mechanisms of Regulation of Gene Expression During Plant Embryo Development.

R. Yadegari, L. Pérez-Grau and R.B. Goldberg. (2001). Seed lectin gene is expressed in different soybean embryo regions during seed development and seed germination. Still in preparation.
L. Pérez-Grau and R.B. Goldberg. (1989). Kunitz Trypsin inhibitor genes are spatially regulated during embryogenesis. Plant Cell 1, 1095-1109.
R.B. Goldberg, S.J. Barker, and L. Pérez-Grau. (1989). Regulation of gene expression during plant embryogenesis. Cell 56, 149-160.
V. Stiefel, L. Pérez-Grau, F. Albericio, E. Giralt, L. Ruiz-Avila, M.D. Ludevid, and P. Puigdomenech. (1988). Molecular cloning of cDNAs encoding a putative cell wall protein from Zea mays and immunological detection of related peptides. Plant Molecular Biology 11, 483-493.
S. Prat, L. Pérez-Grau and P. Puigdomenech. (1987). Multiple variability in the sequence of a family of maize endosperm proteins. Gene 52, 41-49.
L. Pérez-Grau, J. Cortadas, P. Puigdomenech and J. Palau. (1986). Accumulation and subcellular localization of glutelin-2 transcripts during maturation of maize endosperm. FEBS Letters 202, 145-148.

Biophysical Studies on Chromatin Condensation Mechanisms during Euchromatin-Heterochromatin Transitions in Red Cell Differentiation.

J. Bordas, L. Pérez-Grau, M.H.J. Koch, C. Nave, and M. Vega. (1986). A synchrotron radiation study of chromatin superstructure and its condensation mechanism. I. Experimental evidence. European Biophysics Journal 13, 157-173.
J. Bordas, L. Pérez-Grau, M.H.J. Koch, C. Nave, and M. Vega. (1986). A synchrotron radiation study of chromatin superstructure and its condensation mechanism. II. Theoretical analysis and model calculations. European Biophysics Journal 13, 175-185.
L. Pérez-Grau, J. Bordas, and M.H.J. Koch. (1984). A study of chromatin superstructure by synchrotron radiation. X-Ray scattering on solutions and gels. Nucleic Acids Research 12, 2987-2996.

Structural Electron Microscopy and Biochemical Studies on the Reorganization of Chromatin during Spermiogenesis and Red Cell Differentiation.

A. Campos, M.J. Ellison, L. Pérez-Grau, and F. Azorin. (1986). DNA conformation and chromatin organization of a d(CA/GT) sequence cloned in SV40 minichromosome. EMBO Journal 5, 1727-1734.
L. Pérez-Grau, S. Muñoz-Guerra, and J.A. Subirana. (1984). Water soluble polyacrylamide as a novel embedding medium for electron microscopy. Micron 15, 147-151.
F. Azorin, C. Olivares, A. Jordan, L. Pérez-Grau, L. Cornudella, and J.A. Subirana. (1983). Heterogeneity of the histone containing chromatin in sea cucumber spermatozoa. Experimental Cell Research 148, 331-344.
L. Pérez-Grau, F. Azorin, and J.A. Subirana. (1982). Aggregation of mononucleosomes and dinucleosomes into chromatin like fibers. Chromosoma 87, 437-445.
F. Azorin, L. Pérez-Grau and J.A. Subirana. (1982). Supranucleosomal organization of chromatin. Electron microscopy visualization of long polynucleosomal chains. Chromosoma 85, 251-261.
J.A. Subirana, S. Muñoz-Guerra, A. Martinez, L. Pérez-Grau, X. Marcet and I. Fita. (1981). Subunit structure of chromatin fibers. Chromosoma 83, 445-458.

INVITED LECTURES

1999 Fifth National Congress of Plant Molecular Biology, Alicante, Spain.
“Production of Carotenoids and Xantophylls in Brassica Seeds”

1999 First International Congress on Pigments in Food Technology, Seville, Spain.
“Xanthophyll production in Brassica seeds”

1998 Sabanci University, Istanbul, Turkey.
“Aplications of new technologies to plant improvement”

1998 Turkish Research Council, TUBUTAK, Istanbul, Turkey.
Bioinformatics and Genomics Workshop
“Genomic technologies in agricultural biotechnology”

1997 Museum of Science, Barcelona, Spain.
“Agricultural Biotechnology”

1997 Center of Textiles and Technology, CETEMMSA, Mataro, Spain.
“Aplications of Biotechnology in the textile industry”

UNIVERSITY / INDUSTRY RELATIONS

2002-present IRTA and IRTA-Cal, Generatitat de Catalunya’s Department of Agriculture liaison at the University of California, Davis campus.

2001-2 Coordinator of Genomic Research CERES-Seed Institute (UC Davis-U. Utah-UC Los Angeles).

1998 Workshop on the integration of new technologies in a graduate academic program. Sabanci University, Istanbul, Turkey.

1997 Workshop on the “Impact of transgenic plants on agriculture and food”.
Museum of Science, Barcelona, Spain.
Supported by the Spanish Research Council, Spanish Seed Industry, Agrevo, Novartis and Calgene.